photo of a Countess II FL Automated Cell Counter

Accurate cell viability data in 10 seconds

With the option for fluorescence capabilities—brightfield and two user-changeable fluorescence channels—the Invitrogen Countess II FL Automated Cell Counter can count cells, monitor fluorescent protein expression, and measure cell viability in as little as 10 seconds. Countess II FL Automated Cell Counter has a reusable slide option that helps reduce the long-term consumable costs of automated counting significantly. Convenient disposable slides are also available.

  • Fluorescence capabilities—two optional, user-changeable fluorescent channels (>20 fluorescent light cubes to choose from)
  • Reusable slide option—reusable glass slide helps significantly reduce the long-term consumable costs of automated counting

Request a demo Request a customized package quote
Download the Automated Cell Counters brochure

See how easy it is to use the Countess II FL Automated Cell Counter

Fluorescence capabilities—select any two fluorescence colors from 20 light cube options

The Countess II FL instrument has two optional fluorescence channels. The fluorescent colors collected are determined by the insertion of individual light cubes. With 20 light cubes to choose from, the Countess II FL instrument is not limited to detection of GFP- and RFP-expressing cells. Looking to measure the transfection efficiency of cells transduced with an RFP-expression construct with a blue nuclear counterstain? No problem. Simply add the RFP and DAPI light cubes, and you can effectively count the number of cells expressing the RFP fusion within the total population and accurately determine the transfection efficiency.

EVOS LED Light Cubes

Gating based on fluorescence intensity helps enable more accurate counts

The ability to use up to two customizable fluorescence colors with the Countess II FL Automated Cell Counter allows for more accurate counts when dealing with complex samples that contain significant amounts of platelets, RBCs, or debris. In addition, the ability to gate based on fluorescence intensity to determine how many cells are dim vs. bright, or are expressing a fluorescent protein, can save time and effort prior to downstream analysis with techniques such as flow cytometry or high-content analysis (Figure 1).

Gating based on fluorescence intensity. (A) The dim cells (around 50 RFU) have been excluded from the GFP-positive count. (B) Two fluorescence colors are present, each of which can be gated by size, brightness, circularity, and fluorescence intensity.

Figure 1. Gating based on fluorescence intensity. (A) The dim cells (around 50 RFU) have been excluded from the GFP-positive count. (B) Two fluorescence colors are present, each of which can be gated by size, brightness, circularity, and fluorescence intensity.

Read the application note: Fluorescent viability assays on the Countess II FL Automated Cell Counter
Download the application note: Blood cell counting using the Countess II FL Automated Cell Counter

Cell viability assessment is more accurate

Assessing cell viability is a key step in daily cell manipulation, and is required for accurate and efficient downstream processing. With the Countess II FL instrument, there are many fluorescence options in addition to trypan blue staining to quickly and easily check viability prior to downstream sample analysis. Figure 2 shows results obtained using the Invitrogen LIVE/DEAD Viability/Cytotoxicity Kit combined with the EVOS GFP and Texas Red light cubes.

Viability assay using the LIVE/DEAD Viability/Cytotoxicity Kit

Figure 2. Viability assay using the LIVE/DEAD Viability/Cytotoxicity Kit.

Apoptosis and viability, in one count

In addition to cell viability, knowing how many cells are dead or dying are key pieces of information that can be determined quickly with the Countess II FL instrument. Cells of interest can be stained with a viability dye such as Invitrogen SYTOX Red Dead Cell Stain, as well as an apoptosis indicator that measures caspase activation, such as Invitrogen CellEvent Caspase-3/7 Green Detection Reagent. This staining combination, used together with EVOS GFP and Cy5 light cubes, offers a fast and simple method to obtain apoptosis and viability data (Figure 3).

Apoptotic and dead cells counted on a Countess II FL Automated Cell Counter

Figure 3. Apoptotic and dead cells counted on a Countess II FL Automated Cell Counter.

Transfection efficiency and viability, in one count

The ability to assess the number of cells successfully transfected or transduced in a cell population is a basic and critical evaluation parameter. Commonly, transfection or transduction of cells of interest results in the expression of a fluorescent protein reporter, such as GFP. The Countess II FL instrument, loaded with your choice of light cubes, offers a quick and simple method to easily obtain transfection efficiency data (Figure 4).

Determination of transduction efficiency in HeLa cells

Figure 4. Determination of transduction efficiency in HeLa cells.

Reusable slide–now there’s an affordable option for reusable or disposable slides

Countess II FL plus reusable slides equals an end to manual cell counting

The mounting costs of disposable slides and tips have deterred many labs from giving up tedious manual cell counting. In response to this, the Countess II FL instrument was designed to work with a reusable glass slide, which helps significantly reduce the long-term consumable costs of automated counting. Convenient disposable slides are also available.

Learn more about the green features of the Countess II FL reusable slide 
See ordering information for slides

What are other great benefits of automated cell counting?

  • Accuracy—autofocus and auto-lighting minimize user-to-user variability
    • Clumpy cells are not a problem
  • Speed—results in as few as 10 seconds
  • Convenience—built-in dilution calculator and ability to save up to 10 user profiles
    • Built-in dilution calculator
    • Easily gate cells and view histograms
    • Streamline your workflow with custom profiles
    • Printable report with cell counting results

Learn more about automated cell counters

Convince your lab to purchase a Countess II FL Automated Cell Counter

  1. Download and circulate petition
  2. Download and customize prewritten letter
  3. Download application note comparing automated and manual counting
  4. Submit request to PI or lab manager
  5. Ditch your clicker and celebrate
fl-acc

Countess II FL Automated Cell Counter information

Automated Cell Counter – reusable slide, two optional fluorescent channels
The Invitrogen Countess II FL Automated Cell Counter offers brightfield counting and two optional, user changeable fluorescence channels.

 

Performing a Simple Brightfield Count on the Countess II FL Automated Cell Counter
Count cells, measure cell viability and monitor fluorescent protein expression—in just 10 seconds with no slides or tips to buy.

Using the Reusable Hemocytometer on the Countess II FL Automated Cell Counter

Changing the EVOS Light Cubes on the Countess II FL Automated Cell Counter

Technical specifications
  • Processing time: <10 seconds
  • Sample concentration range: 1 x 104–1 x 107 cells/mL
  • Particle diameter range: ~4–60 μm
  • Cell diameter range: ~7–60 μm
  • Required sample volume: 10 μL
  • USB drive: 4 gigabyte, FAT file system
  • Optics: 5 megapixels, 2.5X optical magnification, 3 channels (bright-field plus 2 slots for EVOS LED light cubes)
Physical characteristics
  • Instrument dimensions: 
    9" (W) x 5?" (D) x 9" (H); 228.6 mm (W) X 139.7 mm (D) x 228.6 mm (H)
  • Weight: 8 lbs
Counting area
  • Area: 2.15 mm x 1.62 mm (3.48 mm2)
    graphic showing larger counting area of Countess II FL compared to hemocytometers
Included in the box
  • Countess USB drive for storing and transferring data to your computer
  • Disposable slide holder
  • Reusable slide holder
  • Light cube removal tool
  • Power cord and 4 plug adaptors to accommodate most countries
  • Quick reference card (QRC)

Sold separately:

Researchers use Invitrogen Countess II and Countess II FL Cell Imaging Systems in their labs every day. Here's what some of our users have to say:

"Fast count and nice instruments, easy to use!! I love the small counter!"Dr. Xin Xu, Baylor College of Medicine

"It's very easy to use and it also allows me to quickly update my lab notebook so that each experiment uses the same number of cells. Quick and efficient."Lakeisha Tillery, Meharry Medical College

"It outperformed all other competitor's models that we tried."Michael DaCosta, The Jackson Laboratory

"It's very consistent!"Kim Sylvia, Sanford Burnham Medical Research Institute

"It is very effective at counting cells. It works very well."Mairead Bretney, Sanford Burnham Medical Research Institute

"The Countess II makes it very easy to count cells accurately and quickly - the fact that it tells you the dead cell and live cell count is icing on the cake."Faryal Mir, MIT

"We compared to a hemocytometer and the Countess II always obtained very close results. The fluorescent measurements are a great tool for us to quantify the % transfection and transduction efficiency in our experiments. The light cubes used to detect fluorescence are very easy to switch out when more than two fluorescence sources are needing to be detected. The automatic focusing is very effective and accurate with the option to manually focus."David Waynar, Santa Cruz Biotechnology

"This equipment is very useful for the purposes it is used in our lab. We love it!"Kristin Harwood, BioMarker Strategies, LLC

"Great for a quick check of transfection/transduction efficiency. Easy to use and seems pretty sensitive."Alex Peister, Morehouse College

"Great device. Broad range of cell densities that it can handle. Good at distinguishing clumps and counting cells within clumps."Joanna Rzepka, Veterinary Medical Research & Development

"Low maintenance, fast easy accurate cell counts. Easy for students to use and comprehend."Robert Dean, D'Youville College

"A very accurate cell counter and you can use it to count fluorescent cells as well. Excellent product. I'm very happy with it. I highly recommend it."Massimo Ammirante, University of California, San Diego

The result, image, and data files can be easily exported to a USB drive. The result and image files can be saved as .bmp, .jpg, .tiff, or .png files. The data file is saved as a .csv file that can be easily imported into your current analysis software or viewed in Excel? software.

A comma-separated values (CSV) file stores tabular data (numbers and text) in plaintext form. Plain text means that the file is a sequence of characters, with no data that has to be interpreted as binary numbers. A CSV file can be opened with any third party software or spreadsheet program. The table below describes the categories of the Countess II data saved as a CSV file and opened with a spreadsheet program.

Category Column Name Description
General A Number Sequential sample run number
B File Name Name of file
C Date & Time Date and time of sample run
D Mode BF-Brightfield or FL-Fluorescence
Trypan Blue/
Brightfield
E Total concentration Concentration of the entire sample
F Total cells counted Total number of cells counted in the sample
G Live concentration Concentration of just the “live” portion of the sample
H Live cells counted Total number of “live” cells counted
I Dead concentration Concentration of just the “dead” portion of the sample
J Dead cells counted Total number of “dead” cells counted
K Viability (%) Percent viability of the sample based on trypan blue staining
L Average size (μm) Average cell size in microns
Fluorescence M Cube 1 name EVOS light cube name in the first (top) position
N Cube 1 concentration Concentration of cells showing fluorescence in the first cube position
O Cube 1 (%) Percentage of the total cells in brightfield that show fluorescence in the first cube position
P Cube 1 cells counted Total number of cells counted in the first cube position
Q Cube 2 name EVOS light cube name in the second (bottom) position
R Cube 2 concentration Concentration of cells showing fluorescence in the second cube position
S Cube 2 (%) Percentage of the total cells in brightfield that show fluorescence in the second cube position
T Cube 2 cells counted Total number of cells counted in the second cube position
U Cube 1+2 concentration Concentration of cells showing fluorescence in the first and second cube positions combined
V Cube 1+2 (%) Percentage of the total cells in brightfield that show fluorescence in the first and second cube position combined
W Cube 1+2 cells counted Total number of cells counted in the first and second cube position combined
General Details X Focus value Focal position number
Y BF Light intensity Brightfield light intensity value from 0-100%
Trypan Blue/
Brightfield Count Parameters
Z Live Size min Minimum size of “live” cells in microns
AA Live Size max Maximum size of “live” cells in microns
AB Live Brightness min “Live” adjustment slider value for minimum brightness
AC Live Brightness max “Live” adjustment slider value for maximum brightness
AD Live Circularity “Live” adjustment slider value for circularity
AE Dead Size min Minimum size of “dead” cells in microns
AF Dead Size max Maximum size of “dead” cells in microns
AG Dead Bright min “Dead” adjustment slider value for minimum brightness
AH Dead Bright max “Dead” adjustment slider value for maximum brightness
AI Dead Circ “Dead” adjustment slider value for circularity
Fluorescence Count Parameters AJ Cube 1 Light intensity First (top) light cube light intensity value from 0-100%
AK Cube 2 Light intensity Second (bottom) light cube light intensity value from 0-100%
AL BF Size min Minimum size of “Brightfield” cells in microns
AM BF Size max Maximum size of “Brightfield” cells in microns
AN BF Brightness min “Brightfield” adjustment slider value for minimum brightness
AO BF Brightness max “Brightfield” adjustment slider value for maximum brightness
AP BF Circularity “Brightfield” adjustment slider value for circularity
AQ Cube 1 Brightness min First (top) light cube adjustment slider value for minimum brightness
AR Cube 1 Brightness max First (top) light cube adjustment slider value for maximum brightness
AS Cube 2 Brightness min Second (bottom) light cube adjustment slider value for minimum brightness
AT Cube 2 Brightness max Second (bottom) light cube adjustment slider value for maximum brightness

Counting has been successfully performed on the following cell lines using the Countess II FL Automated Cell Counter:

Cell Animal Organ
A431 Human Skin
A549 Human Lung
Adipocytes Human Adipose-derived stem cells
Aortic smooth muscle Human Smooth muscle
CHO-M1WT2 Chinese hamster Ovary
CHSE Chinook salmon Embryo
COLO-205 Human Colon
COS-7 African monkey Kidney
HeLa Human Cervix
HepG2 Human Liver
HL-60 Human Blood
J774A.1 Mouse Blood
Jurkat Human Blood
K562 Human Bone marrow
MCF7 Human Breast
MCPIP1 Mouse Bone
MRC-5 Human Lung
NIH/3T3 Mouse Embryo
PBMC Human Blood
PC-12 Rat Adrenal gland
Pulmonary artery endothelial Human Blood vessel
Pulmonary artery smooth muscle Human Smooth muscle
Raji Human Lymphocyte
SF-21 Insect Ovary
U266 Human Blood
U2OS Human Bone
Umbilical vein endothelial Human Blood vessel
Whole lysed blood Human Blood

Automated Cell Counter – reusable slide, two optional fluorescent channels
The Invitrogen Countess II FL Automated Cell Counter offers brightfield counting and two optional, user changeable fluorescence channels.

 

Performing a Simple Brightfield Count on the Countess II FL Automated Cell Counter
Count cells, measure cell viability and monitor fluorescent protein expression—in just 10 seconds with no slides or tips to buy.

Using the Reusable Hemocytometer on the Countess II FL Automated Cell Counter

Changing the EVOS Light Cubes on the Countess II FL Automated Cell Counter

Technical specifications
  • Processing time: <10 seconds
  • Sample concentration range: 1 x 104–1 x 107 cells/mL
  • Particle diameter range: ~4–60 μm
  • Cell diameter range: ~7–60 μm
  • Required sample volume: 10 μL
  • USB drive: 4 gigabyte, FAT file system
  • Optics: 5 megapixels, 2.5X optical magnification, 3 channels (bright-field plus 2 slots for EVOS LED light cubes)
Physical characteristics
  • Instrument dimensions: 
    9" (W) x 5?" (D) x 9" (H); 228.6 mm (W) X 139.7 mm (D) x 228.6 mm (H)
  • Weight: 8 lbs
Counting area
  • Area: 2.15 mm x 1.62 mm (3.48 mm2)
    graphic showing larger counting area of Countess II FL compared to hemocytometers
Included in the box
  • Countess USB drive for storing and transferring data to your computer
  • Disposable slide holder
  • Reusable slide holder
  • Light cube removal tool
  • Power cord and 4 plug adaptors to accommodate most countries
  • Quick reference card (QRC)

Sold separately:

Researchers use Invitrogen Countess II and Countess II FL Cell Imaging Systems in their labs every day. Here's what some of our users have to say:

"Fast count and nice instruments, easy to use!! I love the small counter!"Dr. Xin Xu, Baylor College of Medicine

"It's very easy to use and it also allows me to quickly update my lab notebook so that each experiment uses the same number of cells. Quick and efficient."Lakeisha Tillery, Meharry Medical College

"It outperformed all other competitor's models that we tried."Michael DaCosta, The Jackson Laboratory

"It's very consistent!"Kim Sylvia, Sanford Burnham Medical Research Institute

"It is very effective at counting cells. It works very well."Mairead Bretney, Sanford Burnham Medical Research Institute

"The Countess II makes it very easy to count cells accurately and quickly - the fact that it tells you the dead cell and live cell count is icing on the cake."Faryal Mir, MIT

"We compared to a hemocytometer and the Countess II always obtained very close results. The fluorescent measurements are a great tool for us to quantify the % transfection and transduction efficiency in our experiments. The light cubes used to detect fluorescence are very easy to switch out when more than two fluorescence sources are needing to be detected. The automatic focusing is very effective and accurate with the option to manually focus."David Waynar, Santa Cruz Biotechnology

"This equipment is very useful for the purposes it is used in our lab. We love it!"Kristin Harwood, BioMarker Strategies, LLC

"Great for a quick check of transfection/transduction efficiency. Easy to use and seems pretty sensitive."Alex Peister, Morehouse College

"Great device. Broad range of cell densities that it can handle. Good at distinguishing clumps and counting cells within clumps."Joanna Rzepka, Veterinary Medical Research & Development

"Low maintenance, fast easy accurate cell counts. Easy for students to use and comprehend."Robert Dean, D'Youville College

"A very accurate cell counter and you can use it to count fluorescent cells as well. Excellent product. I'm very happy with it. I highly recommend it."Massimo Ammirante, University of California, San Diego

The result, image, and data files can be easily exported to a USB drive. The result and image files can be saved as .bmp, .jpg, .tiff, or .png files. The data file is saved as a .csv file that can be easily imported into your current analysis software or viewed in Excel? software.

A comma-separated values (CSV) file stores tabular data (numbers and text) in plaintext form. Plain text means that the file is a sequence of characters, with no data that has to be interpreted as binary numbers. A CSV file can be opened with any third party software or spreadsheet program. The table below describes the categories of the Countess II data saved as a CSV file and opened with a spreadsheet program.

Category Column Name Description
General A Number Sequential sample run number
B File Name Name of file
C Date & Time Date and time of sample run
D Mode BF-Brightfield or FL-Fluorescence
Trypan Blue/
Brightfield
E Total concentration Concentration of the entire sample
F Total cells counted Total number of cells counted in the sample
G Live concentration Concentration of just the “live” portion of the sample
H Live cells counted Total number of “live” cells counted
I Dead concentration Concentration of just the “dead” portion of the sample
J Dead cells counted Total number of “dead” cells counted
K Viability (%) Percent viability of the sample based on trypan blue staining
L Average size (μm) Average cell size in microns
Fluorescence M Cube 1 name EVOS light cube name in the first (top) position
N Cube 1 concentration Concentration of cells showing fluorescence in the first cube position
O Cube 1 (%) Percentage of the total cells in brightfield that show fluorescence in the first cube position
P Cube 1 cells counted Total number of cells counted in the first cube position
Q Cube 2 name EVOS light cube name in the second (bottom) position
R Cube 2 concentration Concentration of cells showing fluorescence in the second cube position
S Cube 2 (%) Percentage of the total cells in brightfield that show fluorescence in the second cube position
T Cube 2 cells counted Total number of cells counted in the second cube position
U Cube 1+2 concentration Concentration of cells showing fluorescence in the first and second cube positions combined
V Cube 1+2 (%) Percentage of the total cells in brightfield that show fluorescence in the first and second cube position combined
W Cube 1+2 cells counted Total number of cells counted in the first and second cube position combined
General Details X Focus value Focal position number
Y BF Light intensity Brightfield light intensity value from 0-100%
Trypan Blue/
Brightfield Count Parameters
Z Live Size min Minimum size of “live” cells in microns
AA Live Size max Maximum size of “live” cells in microns
AB Live Brightness min “Live” adjustment slider value for minimum brightness
AC Live Brightness max “Live” adjustment slider value for maximum brightness
AD Live Circularity “Live” adjustment slider value for circularity
AE Dead Size min Minimum size of “dead” cells in microns
AF Dead Size max Maximum size of “dead” cells in microns
AG Dead Bright min “Dead” adjustment slider value for minimum brightness
AH Dead Bright max “Dead” adjustment slider value for maximum brightness
AI Dead Circ “Dead” adjustment slider value for circularity
Fluorescence Count Parameters AJ Cube 1 Light intensity First (top) light cube light intensity value from 0-100%
AK Cube 2 Light intensity Second (bottom) light cube light intensity value from 0-100%
AL BF Size min Minimum size of “Brightfield” cells in microns
AM BF Size max Maximum size of “Brightfield” cells in microns
AN BF Brightness min “Brightfield” adjustment slider value for minimum brightness
AO BF Brightness max “Brightfield” adjustment slider value for maximum brightness
AP BF Circularity “Brightfield” adjustment slider value for circularity
AQ Cube 1 Brightness min First (top) light cube adjustment slider value for minimum brightness
AR Cube 1 Brightness max First (top) light cube adjustment slider value for maximum brightness
AS Cube 2 Brightness min Second (bottom) light cube adjustment slider value for minimum brightness
AT Cube 2 Brightness max Second (bottom) light cube adjustment slider value for maximum brightness

Counting has been successfully performed on the following cell lines using the Countess II FL Automated Cell Counter:

Cell Animal Organ
A431 Human Skin
A549 Human Lung
Adipocytes Human Adipose-derived stem cells
Aortic smooth muscle Human Smooth muscle
CHO-M1WT2 Chinese hamster Ovary
CHSE Chinook salmon Embryo
COLO-205 Human Colon
COS-7 African monkey Kidney
HeLa Human Cervix
HepG2 Human Liver
HL-60 Human Blood
J774A.1 Mouse Blood
Jurkat Human Blood
K562 Human Bone marrow
MCF7 Human Breast
MCPIP1 Mouse Bone
MRC-5 Human Lung
NIH/3T3 Mouse Embryo
PBMC Human Blood
PC-12 Rat Adrenal gland
Pulmonary artery endothelial Human Blood vessel
Pulmonary artery smooth muscle Human Smooth muscle
Raji Human Lymphocyte
SF-21 Insect Ovary
U266 Human Blood
U2OS Human Bone
Umbilical vein endothelial Human Blood vessel
Whole lysed blood Human Blood

Countess II FL Automated Cell Counter ordering information

Countess II FL Automated Cell Counter Productivity Package
We provide you a custom quote after selecting the following items:
Request a quote
  • Countess II FL Automated Cell Counter
  • Countess II FL Reusable Slide
  • Countess Cell Counting Chamber Slides
  • Invitrogen Trypan Blue Stain (0.4%)
  • Live/Dead Viability/Cytotoxicity Kit (Green/Red)
  • EVOS Light Cube, GFP
  • EVOS Light Cube, RFP
  • EVOS Light Cube, DAPI
  • 3 years Rapid Exchange Extended Warranty

NOTE: The reusable slide (Cat. No. A25750) is only compatible with the Countess II FL instrument.

*Learn more about Rapid Exchang (REX)
*Learn more about AB Repair Center (ABRC) Support

Service plan overview

  Rapid Exchange (REX) AB Repair Center (ABRC) Support
Plan is designed for Labs that operate under critical timelines Regulated labs that must maintain asset tagging consistency to comply with regulated protocols
Repair time Replacement shipped out in 1 business day Your instrument repaired and returned in ~3 weeks*
Off-site repair service including shipping, parts, and labor ? ?
High-priority telephone and email access to our remote service center ? ?
* For added convenience upgrade to ABRC Support Plus contract, which includes a loan instrument to be shipped within 1 business day.

Learn more about Countess instrument service plans