Invitrogen GeneArt Gene Synthesis

GeneArt Gene Synthesis is a reliable and cost-effective method for obtaining customized DNA constructs with 100% sequence accuracy. Our GeneOptimizer algorithm utilizes a multifactorial approach (up to 20 parameters) beyond just traditional codon optimization to significantly improve protein expression when it comes wild-type protein.

The Instant Designer ordering tool provides a powerful yet simple approach to ordering with both Prime and Value Gene Synthesis Services to tackle everything from simple to complex sequences at affordable prices.

Priority for SARS-CoV-2 projects

As part of Thermo Fisher Scientific's commitment to help accelerate discovery for potential vaccine development, GeneArt Gene Synthesis Services are automatically prioritizing projects once we confirm that the sequences are related to SARS-CoV-2.

For Email orders simply add "Urgent: Coronavirus" in the subject line, and we'll automatically prioritize.

Order your genes with GeneArt Instant Designer

  • Real-time pricing with choice of everyday low price Value and Prime Genes–no promotion code required
  • Express Cloning in Popular Invitrogen Vectors including Gateway and TOPO
  • Single and bulk upload of DNA with GeneOptimizer and Protein sequences
  • Plasmid Prep, Midi–Giga scales

Production times | Compare services | Quick reference guide

Traditional GeneArt Gene Synthesis Portal

  • Upload your own custom vectors
  • Wide selection of vectors
  • Classic GeneArt interface
  • Access previously saved projects ordered using the traditional portal

Order now

Email Ordering

  • Have us configure your project (additional fees for manual processing apply)
  • Project setup by GeneArt scientists
  • Complete the Sequence Submission Form , and then click submit button below

Submit Sequence submission form

Introducing Value and Prime Gene Synthesis Services

With our new Instant Designer gene synthesis tool, we’ve introduced 2 pricing tiers for even better value. Based on your sequence criteria, you’ll have the option to choose within the tool and be guided at each step of the way. Below is a guide to which service level to choose.

  Traditional GeneArt Portal GeneArt Instant Designer
  Traditional Gene Synthesis Services Value Gene Sythesis Services Prime Gene Synthesis Services
Available Vector
  • Standard GeneArt delivery vector with ampicillin or kanamycin resistance markers (pMX, pMA, and pMK)
  • >30 available vectors
  • Upload your own vector
Express cloning Yes Yes Yes
Order method Offline and online Online only Offline and online
Sequence complexity Available for complex and non-complex sequences Non-Complex Sequences (GC content 20–80%, uncomplicated secondary structure and without direct/indirect repeats, or long polypurine/polypyrimidine runs) Available for complex and non-complex sequences
Pricing $$ $ $$
Supported sequence length (bp) 100–12,000 200–5,000 100–9,000
QC sequencing method
  • Sanger Sequencing
  • Next Gen Sequencing
  • Next Gen Sequencing
  • Sanger Sequencing
  • Next Gen Sequencing
Add-on services or upgrades
  • Express, SuperSPEED
  • Sanger Sequencing
  • TSE-Free
Not applicable
  • Express, SuperSPEED
  • Sanger Sequencing
Standard production time Starting from 7 days* Starting from 9 days Starting from 7 days*

* Express and SuperSPEED are available for faster service. For a more detailed see the table below.

Order with confidence

Like all GeneArt Gene Synthesis services, our standard gene synthesis includes the following components to help assure that your construct meets all of your research, quality, and documentation requirements.

  • Configure your project on your own in our online portal
  • Fast and easy project design
  • Instant overview of project history
  • Complete documentation

If you are new to GeneArt Gene Synthesis, check out our online ordering quick start guide and our gene synthesis tutorials.

GeneArt Gene Synthesis production times

It is important to note that genes are made-to-order products and can show variations in producibility, depending on the complexity of the DNA sequence. Typical production times are met for > 90% of genes produced. Production time is now faster by 2 days for genes up to 7 kb and by 9 days for genes between 7–9 kb. Production time is the number of business days required to synthesize genes in our manufacturing facility. Delivery time is in addition to production time and depends on the destination of the shipment.

range (bp)
Prime Gene Synthesis Value Gene Synthesis
Standard* Express* SuperSPEED** Standard
100–200 7-9 6-7 5 N/A
200–1,200 7-9 6-7 5 Starting from 9
1,201–2,000 9-12 8-10 6-7 (up to 1,800bp) 13
2,001–3,000 9-12 8-10 N/A 13
3,001–5,000 Starting from 17 Starting from 15 N/A Starting from 20
5,001–7,000 Starting from 22 Starting from 20 N/A N/A
7,001–9,000 Starting from 22 Starting from 20 N/A N/A
9,001–12,000 Starting from 27 Starting from 25 N/A N/A

* Valid for standard gene synthesis (non-complex, GC content 20-80%, repetitions, secondary structures). For complex sequences, production times will be longer and these will be communicated prior to production.
** Subject to sequence assessment. Order must be placed by 3:00 p.m. CET.
Please note: Rarely, requested sequences are found to be toxic and/or genetically unstable. These production times are only valid for nontoxic sequences that are genetically stable in E. coli.

(Sub) genome and long gene synthesis ordering

For researchers who need long custom DNA constructs for pharmaceutical development (e.g. viral genomes) or for metabolic engineering, pathway engineering, or synthetic biology, we offer a fast, reliable, and economic solution—our proprietary and completely in-house GeneArt DNA synthesis and assembly services.

  • High-throughput  gene synthesis with a capacity of >8.5 Mbp per month (fast and reliable production of DNA pieces for assembly)
  • Expert design and assembly technologies and process expertise for the assembly of constructs from 10 kb to several hundred kilobases, with timelines starting from 25 business days
  • Robust technologies to build DNA constructs with highest complexity
  • Sanger sequencing and next-generation sequencing offer flexible solutions for sequence verification and final QC
  • Proven track record of reliable and timely production for long and complex constructs, with dozens of constructs built each year
  • Proprietary technologies to build high-quality gene fragments up to 3 kb, and assembly kits that enable do-it-yourself construction and assembly

Learn more about (Sub) genome size and long gene synthesis

Why choose GeneArt Gene Synthesis

  • Flexible, custom process: Obtain the exact sequence you need in the vector of your choice—including challenging, complex constructs, and custom vectors.
  • Maximized protein yield: Optional sequence processing with GeneOptimizer software generates sequence variants with enhanced mRNA stability and translational efficiency.
  • Rapid synthesis and assembly: Standard turnaround times start at 10 business days (accelerated options available).
  • Economical: The cost of the GeneArt Gene Synthesis service rivals costs that would be incurred to purchase the oligonucleotides and molecular biology reagents required for gene synthesis, and outsourcing your gene synthesis and cloning means you can focus on your research.
  • Excellent accuracy: All custom synthesized genes are sequenced as part of our ISO 9001:2015–certified quality management system; we only ship constructs with exact sequence agreement.
  • Multiple options: We can synthesize ready-to-clone linear fragments, synthetic genes in circular plasmids, complete vectors, and more. Compare all gene synthesis options 
  • "GeneArt it": Save time and labor by outsourcing your gene synthesis and cloning so you can focus on your research.

Gene synthesis is a fast, simple alternative to traditional cloning

Whether you have limited cloning experience or simply want to save time, the GeneArt Gene Synthesis service helps you move your ideas from the planning stage to the laboratory more quickly. Benefit from our experience in successfully producing over 180,000 constructs for customers as diverse as large pharmaceutical companies, biotechnology start-ups, and basic research institutions. The comparison shown below highlights the time and effort saved compared to traditional cloning.

Why choose GeneArt Gene Synthesis

GeneArt Gene Synthesis process

GeneArt gene synthesis process diagram  

Submitted DNA sequences are designed by our team of highly skilled scientists to include/exclude restriction enzyme sites and translation elements as requested, and are optionally optimized using our GeneOptimizer software for maximum protein production.

Oligonucleotides synthesized in-house, with maximum sequence accuracy, serve as the building blocks for custom gene synthesis.

Genes are assembled using our GeneAssembler process, which facilitates high-throughput gene assembly on an industrial scale for rapid order fulfilment. Using our GeneObserver online tracking system, you can access detailed information 24 hours a day about your order as it progresses through our synthesis process.

Constructs are cloned into one of our standard cloning vectors, or you can specify your own custom vector by adding a subcloning service (additional fees will apply).

All constructs are sequence-verified; only those that match the requested sequence 100% are shipped. The DNA is delivered as 5 μg lyophilized plasmid DNA in microcentrifuge tube.

Quality of every gene construct is controlled as part of our ISO 9001:2015-certified quality management system, which helps ensure reliability and reproducibility. A detailed synthesis report including plasmid map, sequence alignment, and restriction map is provided with each construct.

Up to a 100-fold increase in expression

The GeneOptimizer process has been shown to help deliver large increases in protein yield via a combination of factors that stabilize mRNA and maximize translation efficacy (see figure). Efficacy has been proven in a large comparative gene expression study (Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression Fath et al., 2011 PLoS ONE).

Comparative expression analysis of wild type vs. an optimized human gene

Comparative expression analysis of wild type vs. an optimized human gene. Western blot analysis using α-His antibody. Expression levels of 2 independent transfections per wild type and optimized construct are displayed, showing a 3-fold difference in expression in this particular analysis. Standardization is based on endogenous protein.

Save time with the new express cloning service

Save time with the new express cloning service

Using an alternative production procedure with pre-prepared expression vectors, we can clone your GeneArt synthesized genes directly into selected expression plasmids, saving 4–5 business days compared to the standard workflow with cloning into pMx series vector first.

Turnaround time for expression-ready genes starts at 11 business days (depending on the length of your gene). Adding the gene synthesis express upgrade cuts an additional 2 days, so it is possible to get a turnaround time of 9 business days for your cloned, expression-ready gene.

Learn more about our express cloning service

Sequence length Production time (business days)
Standard** Express** SuperSPEED***
≤1,200 bp 19 17 15
1,201—1,800 bp 22 20 17
1,801—3,000 bp 22 20 NA
3,001—5,000 bp 27 25 NA
5,001—7,000 bp 32 30 NA
7,001—9,000 bp 32 30 NA
>9,000 bp Contact us**** NA

*1,201-7,000bp 2 days faster, 7,001-9,000  bp 9 days faster
**Valid for standard gene synthesis (non-complex, GC content 10–80%). For complex sequences, add 5 business days.
***Subject to sequence assessment. Order must be placed by 3:00 p.m. CET.
****We will respond to your email in a 24hr time frame. Please use our Sequence Submission Form to transfer your sequence.

Please note: Rarely, requested sequences are found to be toxic and/or genetically unstable. These production times are only valid for nontoxic sequences that are genetically stable in E. coli.

Gene Length Production time (business days)
Standard* Express* SuperSPEED***
≤1,200 bp** 19 17 15
1,201**–1,800 bp 24 22 17
1,801–3,000 bp 24 22 n/a
3,001–5,000 bp 29 27 n/a
>5,000 bp Get a quote Get a quote n/a
Complex sequences Get a quote Get a quote n/a

*Valid for standard gene synthesis (non-complex, GC content 10% - 80%).
**In case of GC content of 10% - 20%  or 75% - 80%: 1,000 bp instead of 1,200 bp.
***Subject to sequence assessment. Order must be placed by 3:00 p.m. CET.

Please note: Rarely, requested sequences are found to be toxic and/or genetically unstable. These production times are only valid for nontoxic sequences that are genetically stable in E. coli.

For Research Use Only. Not for use in diagnostic procedures.